生物技术和生物医学档案

开启和关闭状态的高峰SARS-COV-2:与一些整合素结合的关系。生物分子的方法来更好地了解混凝剂的效果

07/02/2021

& lt; h2> Abstract< / h2>

Related the physio-pathological process of COVID-19 disease it is interesting to focus to the aspect.

Played by interaction of Sars-Cov-2 protein with integrins of human epithelial pulmonary cell.

A bio molecular approach help in to deeply verify the involved factors and the results of this Activation RGD mediated.

Of Great interest also the relationship with some vaccine strategy followed by the various pharmaceutical industry.

The results of this work will be useful to think modification in some vaccine increasing the global safety and related some rare ADR.

炔雌醇甲醚半个点击锌(II)酞菁的可控光敏氧化胆固醇

05/07/2021

& lt; p> Abstract< / p>

Four mestranol moieties were chemically linked to Zn(II) phthalocyanine (4) by cycloaddition “Click” reaction using a tetra-azidoethoxy substituted Zn(II)-phthalocyanine (3). The alkyl-azido coupling reaction was realized between azido groups of 3 and alkyl group of mestranol. The alkylation reaction was carried out to obtain cationic Zn(II) phthalocyanine derivative (5). The new compounds were chemically characterized by the known analytical methods. The absorption and fluorescence properties were studied in comparison. The absorption maxima of phthalocyanines 3, 4 and 5 were recorded at approx. shifts of 8 - 12 nm in the far- red region (680 - 684 nm) and the fluorescence maxima (692 - 693 nm) as compared to unsubstituted ZnPc (672 nm, 680 nm) in DMSO. The studies of singlet oxygen generation of 3, 4 and 5 showed relatively high values such as 0.52 for 3; 0.51 for 4 and 0.46 for 5. The fluorescence lifetime of 3.15 ns (3), 3.25 ns (4) and 3.46 ns (5) were determined with lower than the value than for the used standard ZnPc (3.99 ns). The high photo stability was observed for compounds 3, 4 and 5. In addition, the photosensitized oxidation of cholesterol was compared for 3 and 4 with much lower values of oxidation potential than for unsubstituted ZnPc which suggests that the substitution groups influenced on the photooxidation index of the target molecule.

麻提取物的体外有利影响乳头状纤维母细胞定义作为一个抗衰老的候选人

05/05/2021

& lt; h2> Abstract< / h2>

Objective: During aging, skin undergoes structural, cellular and molecular changes, which not only alter skin mechanical properties but also biological and physiological functions. Structurally the epidermis becomes thinner, the dermal epidermal junction flattens and the extra-cellular matrix component of the dermis is disorganized and degraded. The dermis is composed of two compartments: The Reticular dermis is the deepest and thickest part while the upper layer, the papillary dermis, which is much thinner and is in close contact with epidermis, plays an important role in the structure and function of the skin. We have recently shown that the papillary dermis was preferentially affected by skin aging because the activity of fibroblasts in this region was especially altered as a function of age. The purpose of this study was to investigate the capacity of a flax extract as anti-aging component.

Method: We investigated the capacity of a flax extract to stimulate or restore the activity of papillary fibroblasts from young and old donors in cultured monolayers and in reconstructed skin. Several biological markers of extracellular matrix homeostasis and mechanical properties were investigated.

Results: The tested flax extract seemed to improve parameters known to change with age: I/ In monolayers after treatment the number of aged fibroblasts increased II/ In reconstructed skin the flax extract appears to positively regulate some biological activities; particularly in aged fibroblasts where the deposition of laminin 5, fibrillin 1, procollagen I were increased in the dermis and the secretion of specific soluble factors like MMP1, MMP3 and KGF were regulated to levels similar to those observed in young fibroblasts III/ Mechanical properties were improved particularly for elastics parameters (R5, R2 and R7).

Conclusion: The flax extract is a promising anti-aging compound. The treatment of aged papillary fibroblasts resulted in a return to a younger-like profile for some of the studied parameters.

Venomics和antivenomics数据:当前和未来的角度来看

04/20/2021

& lt; h2> Abstract< / h2>

Venom has a very complex and exclusive nature which has been introduced by recent advances in omics technologists. These methods have revealed a new insight into venom studies as venomics. Envenoming by venomous animals is a global concern due to the distribution of important medical species around the world. Treatment of envenomed victims is dependent on accurate and fast identification of animal species with different detection methods. In recent years, new methods have been introduced based on molecular and immunological techniques. Precise diagnosis of species of venomous animals is an essential factor for treatment with specific antivenoms. Venomics and antivenomics data sets help in the selection of specific antivenoms or production of novel antivenoms with greater efficacies.

集成可能最终代谢产物的gc - ms在识别的植酸酶在毕赤酵母属生产Pastoris基于山梨糖醇诱导优化

04/12/2021

& lt; h2> Abstract< / h2>

The isolation of phytase using Pichia Pastoris under methanol/sorbitol co-feeding induction technique was investigated. The biological activity of extracellular phytase after optimization with co-substrates induction in 4 liters fermentor (NBS) increased to 13250 U/ml. This led to a 509 fold increases in comparison to the other type of phytase. This effect was studied via induction with sorbitol/methanol in fermentation by Pichia pastoris GS115 (Mut+) at 20 °C. The interference of by products; methylal, hexamine and (S)-(+)-1,2-propanediol with release of phytase in Pichia pastoris under methanol induction were detected and cannot be repressed by methanol induction alone. The TLC was used for glycerin analysis under methanol/sorbitol induction and the results were lesser compare to that obtained during phytase production under methanol induction alone. This work showed the higher expression of heterologous proteins and by fed batch fermentation; the expression identified an advantage of producing a significant activity of phytase.

Practical applications

Plant derived products including sorbitol have been used as alternative medicines for the therapeutic treatment of various diseases, food supplements and could be used in many manufacturing processes. It serves as a culture media for bacteria, and helps to distinguish the pathogenic E. coli O157:H7 from its most other strains. Cells growing on methanol require high oxygen consumption. Sorbitol was used as an alternative cheap co-feeding for the production of proteins and is a non-repressing carbon source for AOX1 promoter with no effect on the level of r-protein at its induction phase. This report describes the isolation of phytase using Pichia Pastoris under methanol/sorbitol co-feeding induction techniques, and sorbitol showed to be a promising co-substrate, as it could enhance both cell growth and targeted protein productivity. This co-feeding and fed-batch induction technique was used for recombinant phytase production in a small and large scale production and the metabolites were analyzed.

有针对性的一道在哺乳动物细胞的辐射效应:概述

04/12/2021

& lt; p> Abstract< / p>& lt; p>不同波长的辐射可以杀死生物,虽然不同的交互机制取决于他们的能量。理解与活细胞辐射的相互作用是很重要的评估他们的有害影响,并确定他们的治疗潜力。暂时的,这种互动可以大致分为三个阶段,mdash;物理、化学和生物。而辐射会影响所有的重要大分子细胞,尤其重要的是损害其遗传物质,DNA。辐照的后果包括DNA损伤,突变,cross-linkages与其他分子,DNA修复导致染色体畸变和基因表达改变和/或细胞死亡。DNA的突变会导致可遗传的变化和对癌症的感应很重要。虽然这些影响是通过直接交互与目标的辐射,辐射可以与周围的环境导致其间接的行动。辐射的影响不仅在总剂量,也取决于剂量率,让等和细胞类型。 However, action of radiation on organisms is not restricted to interactions with irradiated cells, i.e. target cells alone; it also exerts non-targeted effects on neighboring unexposed cells to produce productive responses; this is known as bystander effect. The bystander effects of ionizing radiations are well documented and contribute largely to the relapse of cancer and secondary tumors after radiotherapy. Irradiation of cells with non-ionizing Ultra-Violet light also exhibits bystander responses, but such responses are very distinct from that produced by ionizing radiations.

Sequence-independent single-primer-amplification (SISPA)作为筛查技术检测意想不到的RNA在细胞培养病毒侵入特工

03/12/2021

& lt; p> Abstract< / p>

The sequence-independent, single-primer amplification (SISPA) enables the random amplification of nucleic acids, allowing the detection and genome sequencing of different viral agents. This feature of SISPA method provides evidence for application of it in monitoring the presence of adventitious RNA viruses in cell cultures. We evaluated SISPA method for the detection of a challenge RNA virus representing adventitious agent in cell cultures. Besides, by optimizing the SISPA method in our laboratory, we found false-positive results on negative control lanes in electrophoresis gels. To investigate the sources of contamination, false-positive results of SISPA were cloned into Escherichia coli cells, sequenced, and phylogenetically analyzed. This data revealed that the SISPA method can be used as an adjunct method to confirm the absence of unexpected adventitious RNA viruses in cell cultures. The phylogenetic analysis of SISPA contaminant sequences showed that the false-positive results were caused by nucleic acid amplification of commercial cDNA synthesis kit reagents, probably tracing back to expression plasmids and host ribosomal sequences, used for the production of enzymes. Therefore, laboratories using random amplification methods must be constantly aware of the potentials of such contaminations, yielding false-positive results and background noise in the final NGS reads.

抗菌素耐药性肺炎克雷伯菌菌株的分离尿液住院病人和门诊病人

02/26/2021

& lt; h2> Abstract< / h2>

Background: Klebsiella pneumoniae is a bacterial species that often causes infections in humans. Infections occur most frequently in hospitalised or immunocompromised patients and are treated with antimicrobials. In recent decades, K. pneumoniae has developed significant resistance to many antimicrobials.

Objective: The main goal of this study was to determine the frequency of resistance of isolated K. pneumoniae strains from urine samples of hospital patients and outpatients, and to find evidence of ESBL strains and their resistance to certain antibiotics.

Methods: During the study period, Klebsiella pneumonia was isolated from the urine samples of 430 patients. The procedure for processing of urine samples, identification, susceptibility toward antimicrobials and evidence of ESBL strains were carried out according to the recommended standards.

Results: Of the total K. pneumoniae isolates, 153 (35.6%) were isolated from hospital patients and 277 (64.4%) from outpatients. Strains isolated from hospital patients were resistant to each tested antibiotic. ESBL strains were detected in 169 (39.30%) samples, 92 (60.13%) from hospital patients and 77 (27.8%) from outpatients.

Conclusion: Strains of K. pneumoniae isolated from the urine of hospital patients and outpatients have developed significant resistance against all tested antibiotic substances. A higher occurrence of ESBL strains was observed in hospital patients than in outpatients. ESBL strains were resistant to all penicillins and almost all cephalosporins. Highly effective antimicrobials were amikacin, colistine, carbapenem and fosfomycin. The best therapeutic results were achieved when patients were treated with fosfomycin and imipenem.

高血压和性相关COVID-19感染死亡率的差异:系统回顾和荟萃分析

12/21/2020

& lt; h2> Abstract< / h2>& lt; p>背景:高血压是心血管疾病和过早死亡的主要原因。高血压发挥了惊人的作用在冠状病毒疾病的死亡率和发病率,以防2019 (COVID-19)感染;然而,大量研究报道相互矛盾的结果。你们;/ p>& lt; p>目的:评估高血压疾病和死亡率的关系COVID-19感染和评估协会上的性别和年龄差异。;/ p>& lt; p>方法:我们已发表的文献进行了系统回顾,发现高血压COVID-19感染和死亡率之间的关系。19篇文章被选中后结构纳入和排除标准系统的回顾和分析。21684医院承认COVID-19患者包括综述和19研究的荟萃分析。大流行的研究涵盖了六个月从2019年12月到2020年5月。;/ p>& lt; p>结果:汇总分析,患者的平均年龄是58岁,男性患者的比例是58.8%。 In contrast, we estimated 33.26% of hypertensive and 19.16% of diabetes mellitus patients in the studies. Hypertension was found to be associated with COVID-19 mortality (“Risk ratio (RR) = 1.45, [95% confidence interval (CI): 1.35 - 1.55]; I2 = 77.1%, p - value
< 0.001”). The association in the meta-regression was affected by sex (p - value = 0.050). The association was found to be stronger in the studies with males ≥ 55% and age ≥ 55 years (“RR = 1.65, [95% CI: 1.52 - 1.78]; I2 = 77.1%, p - value < 0.001”) compared to male < 55% or age < 55 years (“RR = 1.11, [95% CI: 0.94 - 1.28]; I2 = 72.2%, p - value < 0.001”).

Conclusion: Hypertension was significantly strong associated with COVID-19 mortality which may account for the contradiction in the many studies. The association between hypertension and mortality was affected by sex and there were significantly higher fatalities among older male patients.

包装挑战COVID-19药物

11/09/2020

At present Corona virus is the most burning topic across the world. At present there is no drug available to cure 100%. So many companies are trying to make it possible as soon as they can.

评价海洋枯草芽孢杆菌的抗菌、抗癌活动对不同耐药ESRAA3010 Enterococci (MDRE)和癌症细胞系

10/28/2020

& lt; h2> Abstract< / h2>

Fifty nine isolates belonging to six species of Enterococci namely, Enterococcus faecalis, Enterococcus faecium, Enterococcus raffinosus, Enterococcus durans, Enterococcus mundtii and Enterococcus avium (n = 35, 15, 4, 3, 1 and 1 isolates, respectively) were obtained from different clinical specimens including urine, pus, blood, wound, sputum and synovial fluid. The highest numbers of enterococci were recorded from the pus (20 isolates, 33.90%) followed by urine (12 isolates, 20.34%) while the lowest frequency was observed with synovial fluid samples (2 isolates, 3.39%). These isolates showed different multidrug resistant patterns with the lowest resistant for linezolid (n = 5, 8.48%), followed by teicoplanin (n = 14, 23.73%) and vancomycin (n = 20, 33.90%) while they exhibited the highest resistant against penicillin (n = 53, 89.83%), oxacillin (n = 50, 84.75%), erythromycin (n = 49, 83.05%) and streptomycin (n = 47, 79.66 %). On the other hand, a free living marine bacterium under isolation code ESRAA3010 was isolated from seawater samples obtained from the fishing area Masturah, Red Sea, Jeddah, Saudi Arabia. The phenotypic, chemotaxonomic, 16S rRNA gene analyses and phylogenetic data proved that isolate ESRAA3010 is very close to Bacillus subtilis and then it was designated as Bacillus subtilis ESRAA3010. It gave the highest antagonistic activity against all clinical Enterococcus faecalis, Enterococcus faecium, Enterococcus raffinosus, Enterococcus durans, Enterococcus mundtii and Enterococcus avium isolates under study with minimum inhibitory concentration (MIC) ranged from 4 to 56 µg/mL, 4 to 12 µg/mL, 4 to 8 µg/mL, 4 to 8 µg/mL, 8 µg/mL and 4 µg/mL, respectively as well as minimum bactericidal concentration (MBC) (8 to 64 µg/mL, 4 to 16 µg/mL, 4 to 12 µg/mL, 4 to 16 µg/mL, 12 µg/mL and 8 µg/mL, respectively). Moreover it showed anti-proliferative activity against colon (HCT-116), liver (HepG-2), breast (MCF-7) and lung (A-549) carcinomas with IC50 equal to 39, 50, 75 and 19 µg/mL, respectively which indicates its prospective usage in the upcoming decades.

能WondfoA®SARS-CoV-2 IgM /免疫球蛋白抗体作为快速诊断测试?

2020-10-21

Background: An outbreak of novel coronavirus (SARS-CoV-2) disease (COVID-19) has rapidly spread worldwide. The aim of this study was to evaluate and validate the performance of the Wondfo® lateral-flow immunochromatographic assay that detect SARS-CoV-2- IgG, IgM antibodies (Wondfo® IC), using the results obtained by the fluorescence immunoassay test as reference diagnostic.
Material and methods: 97 serum specimens collected and analyzed by four independent laboratories of Sergipe/Brazil was used for validated the Wondfo® SARS-CoV-2 IgM/IgG antibodies test. The COVID-19 positive serum specimens were determined by fluorescence immunoassay technique, used as reference standard.
Results: An overall of 97 serum specimens show 39 (39/97) SARS-CoV-2 IgG positive specimens, 33 (33/97) SARS-CoV-2 IgM positive specimen and 25 non-reagent specimens (25/97). However, the Wondfo® IC assay detected only 9 (9/97) IgM/IgG positive specimen and 25 (25/97) no-reagent specimen. A weak correlation was found between the outcomes of the Wondfo® IC assay and fluorescence test. The accuracy between the two tests was 32.08%. The sensitivity, specificity, positive predictive value, and negative predictive value of Wondfo® IC assay were of 11.12%, 100%, 100% and 25.27%, respectively. Moreover, no false positive sample was determinate, whereas 88.89% of false negative results were found.
Conclusion: The Wondfo® IC test failed in providing a quick, valid, and reliable results and appears not to be a good alternative for clinical use in detecting pandemic coronavirus. However, if the limitations of the rapid test are known, some correction factors can be used in order to adjust the epidemiological data.

统计和COVID-19方程模型分析

07/03/2020

& lt; h2> Abstract< / h2>

Background: An infectious disease caused by a novel coronavirus called COVID-19 has raged across the world since December 2019. The novel coronavirus first appeared in Wuhan, China, and quickly spread to Asia and now many countries around the world are affected by the epidemic. The deaths of many patients, including medical staff, caused social panic, media attention, and high attention from governments and world organizations. Today, with the joint efforts of the government, the doctors and all walks of life, the epidemic in Hubei Province has been brought under control, preventing its spread from affecting the lives of the people. Because of its rapid spread and serious consequences, this sudden novel coronary pneumonia epidemic has become an important social hot spot event. Through the analysis of the novel coronary pneumonia epidemic situation, we can also have a better understanding of sudden infectious diseases in the future, so that we can take more effective response measures, establish a truly predictable and provide reliable and sufficient information for prevention and control model.

Methods: We establish different models according to the different developments of the epidemic situation, different time points, and different response measures taken by the government. To be specific, during the period of 2020.1.23-2020.2.7, the traditional SIR model is adopted; during the period of 2020.2.8-2020.3.30, according to the scientific research results, it was considered that the novel coronary pneumonia has a latent period, so in the later phase of epidemic development, the government has effectively isolated patients, thus we adopt the SEIQR model accordingly. During the period of 2020.3.31-2020.5.16, because more asymptomatic infected people were found, we use the SEIQLR model to fit. Finally, through a SEIR simulator, considering the susceptible number, the latent number, the infected number, the cured number, death number and other factors, we simulate the change of various numbers of people from the beginning to the next 180 days of novel coronary pneumonia.

Findings: The results based on the analysis of differential equations and kinetic models show that through the prediction of the model established in the first phase, the epidemic situation of novel coronary pneumonia in Hubei Province was controlled at the end of March, which is in line with the actual situation. The rest of Hubei province, except for Wuhan, lifted control of the departure channel from 0:00 am on March 25, and Wuhan was also unblocked on April 8. Through the establishment of the second-phase model, it is found that the epidemic situation will reach its peak in mid-February. For example, the quarantine admission of the hospital declined after mid-February, which is inseparable from the measures to build square cabin hospitals in early February so that more and more patients can be admitted. The model established in the third phase shows that the epidemic had been completely controlled by the end of May, which is also in line with the reality. Because in mid-May, the Wuhan government conducted a nucleic acid test on all the citizens to screen for asymptomatic infected persons to fundamentally control the spread of novel coronary pneumonia.

Interpretation: Hubei Province, as the center of the initial outbreak of novel coronary pneumonia, people were forced to be isolated at home during the Spring Festival, the most important Chinese holiday, and the whole society was in a state of suspension of work and study. The Chinese government had taken many measures in response to the epidemic, such as shutting down the city, vigorously building square cabin hospitals, and prohibiting people from gathering. At the beginning of May this year, the epidemic in Hubei Province was finally effectively controlled. For ordinary citizens, we should not cause unnecessary panic about the unknown novel coronavirus. Instead, we should fully understand and be familiar with this virus. In addition to the relevant medical knowledge, we should also understand the spread of infectious diseases through appropriate mathematical models. By mathematical models, we can understand the degree of harm of infectious diseases, when to control it, how to stop it, and use scientific views to reveal the original face of the novel coronavirus to the public without causing social panic.

进步的果实的化学成分及生物活性Amomi

03/13/2020

& lt; h2> Abstract< / h2>

Fructus Amomi is “one of the top four south authentic Traditional Chinese Plant Medicines”, widely distributed in tropical and subtropical districts of China, such as Fujian, Guangdong, Guangxi, Hainan, etc., which has ever been used in clinical for treatment of the digestive diseases. In addition, it has also been used as spices. In recent years, some research about its components together with pharmacological activities have been reported. In this Mini review, we mainly describe the recent progress of the components and pharmacological activities of Fructus Amomi, to provide the reference for the relevant researchers.

系统发育分析metalloprotease从转录组的毒液腺Hemiscorpius lepturus

02/05/2019

& lt; h2> Abstract< / h2>

Hemiscorpius lepturusis a dangerous scorpion and referred to health concern issue in Khuzestan, Iran. The venom of H.lepturus is cytotoxic and its effect is similar to spider Loxosceles reclusa. Metalloproteinases are the important class of enzymes in the venom that has hemorrhagic activity. The early finding suggests the existence of metalloproteases in the transcriptome of venom gland of H.lepturus. Phylogenetic analysis was accomplished to reveal the evolutionary relationship of identified metalloproteases. The phylogenetic tree was constructed by Molecular Evolutionary Genetics Analysis software and neighbor-joining method. Results showed among three sequences, two metalloproteinases named HLMP1 and HLMP3 of H.lepturus were most close to spider P. tepidariorum. The third sequence named HLMP2 was different and formed an independent clade in the phylogenetic tree. The results suggest that the sequence of metalloproteases in the venom component of H.lepturus is similar to the spider than the scorpion.

细胞核周围的光环在子宫颈抹片检查显示阴道毛滴虫

01/11/2019

& lt; h2> Abstract< / h2>

Trichomonas vaginalis could be seen in Pap smears where it is reported, but because main concerned is placed on malignant cells in Pap smears, not much effort is done to search for this parasite in smears. In this study, 100 cervical and vaginal specimens were examined microscopically by the conventional Papanicolaou method and liquid base cytology (LBC) for the presence of Trichomonas vaginalis (T.vaginalis). 16% were infected with T.vaginalis while 10% of diagnosis based on both perinuclear halo and T. vaginalis presence although the association between perinuclear halo and T, vaginalis is statistically insignificant it is recommended to increase the number of the cases to prove or disprove the association.

可食用的家禽疫苗对抗传染性法氏囊病

11/09/2018

Editorial

Poultry industry is a domineering section of agriculture sector in the world as it provides meat, income and employment. Of the poultry industry, broiler chicken is dominating, as US export was more than 41 billion pounds of chicken (about 16.5 percent of production) in 2017 [1]. In Pakistan, the poultry industry contributes around 1.4 percent to the GDP and 31 percent to total meat production [2]. The global demand for this meat is rising in developing world including Pakistan. To meet the needs, rearing of poultry at both domestic and commercial levels is imperative [3]. However, the industry faces a lot of constraints, preventing it from reaching its maximal potential. Poor welfare, insufficient quality nutrition and devastating diseases are some of these problems. Amongst the myriad of diseases, Infectious Bursal Disease (also known as Gumboro disease, infectious avian nephrosis and infectious bursitis) is an acute, contagious viral disease of chicken [4-6]. It not only affects domesticated but also wild poultry throughout the world by targeting the immune system. [7-9].

Gumboro disease is caused by Infectious Bursal Disease Virus (IBDV), which is a member of genus Avibirnavirus of the Birnaviridae family. IBDV is a non-enveloped virus having a capsid that contains a double stranded RNA genome, split into two segments A and B [10]. Segment A of the genome encodes four proteins (VP2, VP3, VP4 and VP5) while segment B encodes VP1. Based on Pathogenicity the IBDV strains can be grouped into three pathotypes; classical virulent, antigenic variant and very virulent [11]. Though the first outbreak of very virulent IBD virus was reported in Europe in early 1990s [12], recent pandemics across Asia, Africa and South America [13] have heavily damaged commercial and wild poultry. Contamination of a rearing site with the IBDV leads to a horizontal transmission of disease across the flocks through contaminated feed and water. However, vertical transmission is not reported yet [14]. Classical virulent strains flaunt a mortality rate as high as 20-30%, mostly due to the widespread bursal damage in infected poultry [15]. Classical virulent strains are the source of commercially available vaccines against IBD viral infections. In early 1980s, new antigenic variants emerged, causing more than 50 percent mortality in birds by rapid bursal damage [16]. Current vaccines prepared from classical strains have failed to control the disease caused by these antigenically variant strains. In the late 1980s, very virulent IBD virus strains were reported that induced IBD with a more pronounced bursal lesion and higher mortality (up to 90 percent) when compared to other IBDV strains [17]. In addition, vaccination against the very virulent strains encounters many challenges as very virulent IBD virus can breakthrough protective antibodies, and therefore requires more efficient vaccination approaches.

氯化钾的影响(rpm /热)在大肠杆菌细菌蛋白酶生产,铜绿假单胞菌和粪大肠

09/11/2018

& lt; h2> Abstract< / h2>

Background: Proteases are a group of enzymes that catalyze the cleavage of peptide bonds in proteins found in nature. Microbial protease constitutes one of the most important for industrial aplications. Proteases play a crucial role in numerous pathologic processes as well. KCl is an unnatural salt. The purpose of this study was to examine the effect of this salt on protease production under different agitation and heat conditions.

Methods: The effects of KCl (rpm/heat) on the production of a protease, of E. coli, P. aeruginosa and E. faecalis strain, were investigated. The decrease in protease production at 37 °C was also observed in this work that proved that heat plays a major role in enzyme production.

Results: The presence of KCl also caused a decrease in protease production in three bacterial species. The use of KCl appears to be a viable alternative when it is necessary to reduce protease activity outside of industrial applications (such as health care). This unique property makes it attractive and useful to be used in health industries. In the future we think that it will contribute to clarification of the matter in this way.

重大影响维生素K在不同代谢疾病根据积极作用的维生素D水平,和钙

04/05/2018

& lt; h2> Abstract< / h2>

Vitamin K was discovered as nutrient of blood clotting. There are two main types of vitamin K, vitamin K1, and vitamin K2. Although the structure of vitamin k is stable but also, there are differences between them. Vitamin k1 is mainly present in dark green leafy plant; while vitamin k2 present in animal and fermented plant. There are different sources of vitamin k. The normal human body is required about 200µg/day from vitamin k in both forms. Vitamin k1 can easily converted into vitamin k2.There is big difference between work of types of vitamin k, where each one responsible for special job. Vitamin k play important role in various metabolic process; vitamin k especially k2 play important role in protect the body from heart attack , in addition to reduce the development of osteoporosis and bone disease in combination with vitamin D and calcium. Vitamin k responsible for increase the secretion of male sex hormone and infertility. Vitamin k is consider one of the most important vitamin, it can save human from death as result of precipitation of calcium on arteries. Due to the importance of vitamin k, this article will discuss vitamin k, its presence, and role in different diseases.

ABC和MFS转运蛋白:抗真菌耐药性的原因

01/05/2018

& lt; h2> Abstract< / h2>

Fungi cause a variety of diseases and are difficult to treat owing to their eukaryotic nature resulting in dearth of antifungal targets at hand. This problem is further elevated many folds due to the resistance mechanisms of fungi through which they circumvent the antifungal drugs administered for therapeutic purposes. Fungi have a variety of strategies for obtaining these resistances, amongst them pivotal role is played by the ABC and MFS transporters. This article encompasses the important genes and their respective roles of both the classes of the transporters in different species of fungi.

利弊的争议在分子成像和动态双-标准DNA / RNA的人类保存干细胞Cells-Binding纳米分子与雄激素/合成类固醇(AAS)或睾酮衍生品通过跟踪氦- 4核使用同步辐射(粒子)

11/15/2017

在目前的研究中,我们调查了利弊的争议上的双重标准DNA / RNA分子成像和动态的人类保存干细胞cells-binding纳米分子与雄激素/合成类固醇(AAS)或睾酮衍生品通过跟踪氦- 4核使用同步辐射(粒子)。在这方面,双重标准的酶氧化DNA / RNA的人类保存干细胞cells-binding纳米分子血红素氧化酵素(或血红素氧化酵素),如辣根过氧化物酶(HPR) Chloroperoxidase (CPO),乳过氧化物酶和木质素过氧化物酶(唇)是一个重要的过程从合成和机械的观点。

三个不同的牡蛎蘑菇的营养价值香蒲草衬底上生长

08/31/2017

三个不同的平菇菌株包括侧耳属佛罗里达(PF),侧耳属eous (PE)和侧耳属sajor-caju (PS)香蒲草衬底上成功培养。比较分析不同的参数即。,生物效率(是)和蛋白质、碳水化合物、粗纤维和脂肪含量在子实体进行评估。According to biological efficiencies obtained PF (90%) was superior strain, while order can be represented as 90%> 89%> 82% respectively in PF>PS>PE. The highest protein (4.8 g), lipid (0. 61 g) and dietary fiber (31.6g) values were obtained in the fruitings of Pleurotus sajor-caju. However, the utmost level of carbohydrate (41g) was recorded in Pleurotus eous. On the basis of the observation of the present study we recommend use of cattail weed substrate for cultivation of oyster mushrooms for better nutrients.

最好是先进的耦合方法安装若微粒?

07/17/2017

微粒子(MPs)被认为是重要的诊断生物标记在许多疾病有前途的预测价值。目前有几种方法,用于检测议员的数量和特征的结构和特征。因此,议员检测方法一直保持相当昂贵和耗时。审查被描述视角使用耦合议员测定和结构分析的方法。确实有大量的证据对原子力显微镜的结合或耦合(NTA)与微纳米粒子跟踪分析,等离子体共振法和荧光量子点可以表现出更准确的检测数量和结构能力的议员相比,传统的流式细胞仪和荧光显微镜。几种组合方法是否有用的高级议员检测尚不完全清楚,虽然它非常有前途。

简洁回顾:考虑制定、交付和管理生物制药的路线

06/28/2017

& lt; h2> Abstract< / h2>

The drugs of biological origins have attracted the attention of many pharmaceutical companies where it is essential to protect the heterogeneous nature and the optimal three dimensional structures of the different macromolecules. These molecules are used in both the investigation and therapy purposes, so their maximum activities should be maintained. This requires the designing of certain delivery formulations that suits the macromolecule nature, its target organ, the required dose and delivery route, and that’s why the biotech companies invest millions of dollars towards achieving that. The first main focal point of this article includes the recent developments in the formulation technologies for several biomacromolecule classes. The second focal point concentrates on the current considerations for optimizing their delivery for a maximum performance in the body.

细菌Heterotrimeric Amidotransferase GatCAB:功能、结构和系统抑制剂

05/01/2017

& lt; h2> Abstract< / h2>

tRNA-dependent amidotransferases (AdT) are essential enzymes for protein biosynthesis in many bacteria and in all archaea. As AdT is essential for a number of pathogenic bacteria, and it is absent from mammalian cytoplasm, it is considered as a putative target for novel inhibitors that could be lead compounds to develop a new class of antibiotics. Besides GatFAB of Saccharomyces cerevisiae mitochondria and GatAB of Plasmodium falciparum apicoplast, all reported AdT can be divided into two groups: heterodimeric GatDE and heterotrimeric GatCAB. The latter is required to catalyze the conversion of Glu-tRNAGln and/or Asp-tRNAAsn into Gln-tRNAGln and/or Asn-tRNAAsn in many pathogenic bacteria. Recently determined high resolution crystal structures of several GatCAB could be used to design new inhibitors. In this review, we highlight the essential role of AdT for the faithful translation of glutamine and/or asparagine codons, we describe important features of the crystal structures of several GatCAB as well as tRNA/AdT/aaRS complexes for the formation of Gln-tRNAAsn and Asn-tRNAAsn, we finally summarize discoveries of AdT inhibitors based on their analogy to glutamine, adesosine tripoliphosphate and 3’-end of tRNA.

生物仿制药的大胆

05/01/2017

Letter to Editor

The so-called “biotechnological revolution” is changing the structure of the Pharmacopoeia [1]. The space of biological products, previously limited to blood products and vaccines, grew from the introduction of first recombinant therapeutics in the 1980s until attaining a 25% by value of the pharmaceutical market. This share is expected to reach 50% in the coming years. More than 80 biotechnology drugs have entered the market in the last ten years. It is estimated that there are more than 900 biological products on development for more than 100 diseases [2].

干扰素的概述:特征、信号和抗癌的效果

04/25/2017

& lt; h2> Abstract< / h2>

Interferons are multifunctional cytokines widely used in clinical settings as an anti-viral drug. In addition, interferon’s exhibit anti-cancer and anti-bacterial effects. Nearly two thousand papers related to interferon are published each year, which illustrates the importance placed by researchers on the study of interferon. This review focuses on recent advances in the study of interferon, particularly in the areas of its mechanism of anti-cancer effect and signal transduction. We also describe the tumor resistance to interferon and the side-effect of interferon-based therapy, which leads to an expectation of future research of interferon.